Abstract: Objective  To understand the detection rates and the drug sensitivities of Pseudomonas aeruginosa and Acinetobacter baumannii in tuberculosis (TB) patients, to provide the basis for rational use of antibiotics and control of nosocomial infection. Methods  According to the National clinical laboratory operating procedures, 6493 specimens (including 5059 sputa, 878 fiberoptic bronchoscopy lavage fluid (FBLF), and 556 thoracic or abdominal cavity effusion, wound secretions, blood etc.) were collected from clinical laboratory in the Guangzhou Chest Hospital from January in 2011 to August in 2013,and then the bacteria were identified and performed the drug sensitivity test by PHOENIX-100 automatic microorganism analyzer.   Results There were 3612 strains isolated from 6493 specimens, including 985 (27.27%) Pseudomonas aeruginosa and 324 (8.97%) Acinetobacter baumannii. Of 985 Pseudomonas aeruginosa isolates, 100.00% were sensitive to polymyxin, 94.52%（931/985）were sensitive to tobramycin, 99.39% (979/985) were resistance to ampicillin/Shubatan, and cefazolin. 100.00%（324/324）Acinetobacter baumannii isolates were highly sensitive to polymyxin, and different degrees of resistance to other antibiotics.  Conclusion Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates were resistance to various antibiotics. Should be reasonable to select antibiotics according to the drug sensitivity test results, to avoid drug-resistant strains.

Key words: Tuberculosis, Pseudomonas aeruginosa, Acinetobacter baumannii, Microbial sensitivity tests